Question of the Day

Ok, here is my question for the day, it is something I have been wondering about for a while now. Hashem punished Adam and Eve for eating of the fruit of the Tree of the Knowledge of Good and Evil. My question is, how can they be held accountable for eating of the fruit? I am assuming that before they ate from the Tree of Knowledge of Good and Evil, they didn't know the difference between good and evil. So how would they have known that disobeying Hashem was wrong?